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igg1  (SouthernBiotech)


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    SouthernBiotech igg1
    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 <t>(IgG1);</t> (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).
    Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+igg1/Mouse+Anti-Human+IgG1+Hinge-FITC/pmc12934314-48-54-57
    Average 93 stars, based on 22 article reviews
    igg1 - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration"

    Article Title: Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration

    Journal: Journal of Translational Autoimmunity

    doi: 10.1016/j.jtauto.2026.100359

    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).
    Figure Legend Snippet: Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Techniques Used: Immunofluorescence, Fluorescence, Control

    Related Articles

    Incubation:

    Article Title: Multiparametric optimization of human primary B-cell cultures using Design of Experiments
    Article Snippet: .. 0.3 μg/mL Donkey anti-human IgG (Jackson ImmunoResearch, 709-005-098), 2.5 μg/mL Mouse Anti-human IgG1 (SouthernBiotech, 9052-01), 1 μg/mL Mouse Anti-human IgG3 (SouthernBiotech, 9210-01), 1 μg/mL Goat Anti-Human IgA (SouthernBiotech, 2053-01), or 2.5 μg/mL anti-human IgE (SouthernBiotech, 9240-01), all incubated o/n at 4 °C. .. Wells were emptied and blocked with 200 μL TBS (Tris-buffered saline (137 mM NaCl, 2.7 mM KCl, 25 mM Tris/Tris-HCl) containing 0.09% (w/v) sodium azide (Fisher Scientific, BP2471-500)) with 1% BSA (Sigma A 4503) for 1 hour at RT and washed 3 times using TBS with 0.05% (v/v) Tween-20 (TBS/TW).

    Article Title: BA.1 breakthrough infection elicits distinct antibody and memory B cell responses in vaccinated-only versus hybrid immunity individuals
    Article Snippet: After incubation, 558 the plates were centrifuged and washed with PBS-0.05% Tween 20 using a magnetic Bio-Plex 559 Pro wash station (Bio-Rad, #30034376). .. 560 For IgG subtyping, mouse anti-human IgG1, IgG2, IgG3, IgG4 antibodies conjugated with 561 phycoerythrin (PE, Southern Biotech) were added at a final concentration of 1.3 μg/mL and 562 the mixture was incubated for 2h at room temperature on a plate shaker. .. For the detection 563 of antigen-specific FcγR binding, soluble recombinant FcγRIIa (higher affinity polymorphisms 564 FcγRIIa-H131) and FcγRIIIa (higher affinity polymorphisms FcγRIIIa-V158) dimers were 565 biotinylated and added at a final concentration of 1.3 μg/mL.51 After incubation at room 566 temperature for 2h on a plate shaker, the plate was washed with PBS-0.05% Tween 20, and 567 streptavidin-PE conjugate (SAPE, Invitrogen) was added at 1 μg/ml for another 2h incubation 568 time on a plate shaker.

    Article Title: BA.1 breakthrough infection elicits distinct antibody and memory B cell responses in vaccinated-only versus hybrid immunity individuals
    Article Snippet: After incubation, the plates were centrifuged and washed with PBS-0.05% Tween 20 using a magnetic Bio-Plex Pro wash station (Bio-Rad, #30034376). .. For IgG subtyping, mouse anti-human IgG1, IgG2, IgG3, IgG4 antibodies conjugated with phycoerythrin (PE, Southern Biotech) were added at a final concentration of 1.3 μg/mL and the mixture was incubated for 2h at room temperature on a plate shaker. ..

    Concentration Assay:

    Article Title: BA.1 breakthrough infection elicits distinct antibody and memory B cell responses in vaccinated-only versus hybrid immunity individuals
    Article Snippet: After incubation, 558 the plates were centrifuged and washed with PBS-0.05% Tween 20 using a magnetic Bio-Plex 559 Pro wash station (Bio-Rad, #30034376). .. 560 For IgG subtyping, mouse anti-human IgG1, IgG2, IgG3, IgG4 antibodies conjugated with 561 phycoerythrin (PE, Southern Biotech) were added at a final concentration of 1.3 μg/mL and 562 the mixture was incubated for 2h at room temperature on a plate shaker. .. For the detection 563 of antigen-specific FcγR binding, soluble recombinant FcγRIIa (higher affinity polymorphisms 564 FcγRIIa-H131) and FcγRIIIa (higher affinity polymorphisms FcγRIIIa-V158) dimers were 565 biotinylated and added at a final concentration of 1.3 μg/mL.51 After incubation at room 566 temperature for 2h on a plate shaker, the plate was washed with PBS-0.05% Tween 20, and 567 streptavidin-PE conjugate (SAPE, Invitrogen) was added at 1 μg/ml for another 2h incubation 568 time on a plate shaker.

    Article Title: BA.1 breakthrough infection elicits distinct antibody and memory B cell responses in vaccinated-only versus hybrid immunity individuals
    Article Snippet: After incubation, the plates were centrifuged and washed with PBS-0.05% Tween 20 using a magnetic Bio-Plex Pro wash station (Bio-Rad, #30034376). .. For IgG subtyping, mouse anti-human IgG1, IgG2, IgG3, IgG4 antibodies conjugated with phycoerythrin (PE, Southern Biotech) were added at a final concentration of 1.3 μg/mL and the mixture was incubated for 2h at room temperature on a plate shaker. ..

    other:

    Article Title: Multiparametric Optimization of Human Primary B-Cell Cultures Using Design of Experiments.
    Article Snippet: ELISA was performed on dilutions of culture supernatant in duplicates using the following kits: Human BAFF/ BLyS/TNFSF13B DuoSet ELISA (#DY124- 05), Human IL- 4 DuoSet ELISA (#DY204- 05) and Human IL- 21 DuoSet ELISA (#DY8879- 05) (all from R&D Systems) according to the manufacturer's instructions.



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    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 <t>(IgG1);</t> (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).
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    Image Search Results


    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Journal: Journal of Translational Autoimmunity

    Article Title: Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration

    doi: 10.1016/j.jtauto.2026.100359

    Figure Lengend Snippet: Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Article Snippet: The reactivity of the patients’ sera and/or CSF against the retina was evaluated retrospectively with frozen samples (−80°, EXPLAINEUR biobank) through an indirect immunofluorescence technique using sections of monkey retina (Ref. FA1172-1005, Euroimmun), detected with an FITC-labelled secondary antibody anti-human IgAGM (Euroimmun conjugate) or directed against IgA (ref. F0204, DAKO), IgM (ref. F0203, DAKO), IgG1 (ref. 9052-02, Southern Biotech) and IgG4 (ref. 9200-02, Southern Biotech).

    Techniques: Immunofluorescence, Fluorescence, Control